Lysozyme Crystal Growth Photographs


 
Colored dye was used to indicate the various salt concentrations
% salt/colors from left to right

2.0 % salt - pale green
2.5 % salt - pink
3.0 % salt - yellow
3.5 % salt - blue
4.0 % salt - rose
4.5 % salt - light purple
5.0 % salt - dark blue green
5.5 % salt - red wine

2.0 % salt (pale green color) -
no crystals
 
2.5 % salt (pink color) - 
no crystals
 
3.0 % salt (yellow color) -
no crystals
 
 3.5 % salt (blue color)-
nice orthorhombic or tetragonal crystal shape -  crystal size about .1 mm

* see comment below right

4.0 % salt (rose color) -
nice orthorhombic or tetragonal crystal shape - crystal size about .05 mm

* see comment below right

4.5 % salt (light purple color) -
small crystals formed but no good photographs were available
 *Salt concentration has little to do with the morphology of the crystal.  If the crystals are grown above 25 degrees C (77 degress F), the predominant form is orthorhombic (longer aspect ratio).  Below 20 degrees C (68 degrees F), the predominant form is tetragonal (shorter aspect ratio).  In between, the form is usually teragonal which sometimes interconverts to orthorombic.  The only way to tell for sure is to do an xray diffraction.
(Anna Holmes; Univ of Alabama at Huntsville)
5.0 % salt (dark blue green color) - many small crystals with undetermined crystal shape
 5.5% salt (wine color) - 
many small crystalline to amorphous particles
Photographs were taken with a Sony  Mavica (MVC-FD7) digital camera off  of a TV screen.
The crystal images were projected on to the TV screen by a camera attached to a  microscope.


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Created on August 6th, 1999 by Robert S. Smith